A 27-Amino-Acid Deletion in the Neuraminidase Stalk Supports Replication of an Avian H2N2 Influenza A Virus in the Respiratory Tract of Chickens
Identifieur interne : 000F94 ( Main/Exploration ); précédent : 000F93; suivant : 000F95A 27-Amino-Acid Deletion in the Neuraminidase Stalk Supports Replication of an Avian H2N2 Influenza A Virus in the Respiratory Tract of Chickens
Auteurs : Erin M. Sorrell [États-Unis] ; HAICHEN SONG [États-Unis] ; Lindomar Pena [États-Unis] ; Daniel R. Perez [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Appareil respiratoire (virologie), Caille, Coturnix, Données de séquences moléculaires, Délétion de séquence, Grippe chez les oiseaux (virologie), Lignée cellulaire, Maladies de la volaille (virologie), Poulets, Protéines virales (génétique), Protéines virales (métabolisme), Réplication virale, Sialidase (génétique), Sialidase (métabolisme), Sous-type H2N2 du virus de la grippe A (enzymologie), Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (physiologie).
- MESH :
- enzymologie : Sous-type H2N2 du virus de la grippe A.
- génétique : Protéines virales, Sialidase, Sous-type H2N2 du virus de la grippe A.
- métabolisme : Protéines virales, Sialidase.
- physiologie : Sous-type H2N2 du virus de la grippe A.
- virologie : Appareil respiratoire, Grippe chez les oiseaux, Maladies de la volaille.
- Pascal (Inist)
- Wicri :
- topic : Vétérinaire.
English descriptors
- KwdEn :
- Animals, Avian influenzavirus, Cell Line, Chicken, Chickens, Coturnix, Deletion, Influenza A Virus, H2N2 Subtype (enzymology), Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (physiology), Influenza A virus, Influenza in Birds (virology), Molecular Sequence Data, Mutation, Neuraminidase (genetics), Neuraminidase (metabolism), Poultry Diseases (virology), Quail, Replication, Respiratory System (virology), Respiratory tract, Sequence Deletion, Veterinary, Viral Proteins (genetics), Viral Proteins (metabolism), Virus Replication.
- MESH :
- chemical , genetics : Neuraminidase, Viral Proteins.
- enzymology : Influenza A Virus, H2N2 Subtype.
- genetics : Influenza A Virus, H2N2 Subtype.
- chemical , metabolism : Neuraminidase, Viral Proteins.
- physiology : Influenza A Virus, H2N2 Subtype.
- virology : Influenza in Birds, Poultry Diseases, Respiratory System.
- Animals, Cell Line, Chickens, Coturnix, Molecular Sequence Data, Quail, Sequence Deletion, Virus Replication.
Abstract
The events and mechanisms that lead to interspecies transmission of, and host adaptation to, influenza A virus are unknown; however, both surface and internal proteins have been implicated. Our previous report highlighted the role that Japanese quail play as an intermediate host, expanding the host range of a mallard H2N2 virus, A/mallard/Potsdam/178-4/83 (H2N2), through viral adaptation. This quail-adapted virus supported transmission in quail and increased its host range to replicate and be transmitted efficiently in chickens. Here we report that of the six amino acid changes in the quail-adapted virus, a single change in the hemagglutinin (HA) was crucial for transmission in quail, while the changes in the polymerase genes favored replication at lower temperatures than those for the wild-type mallard virus. Reverse genetic analysis indicated that all adaptive mutations were necessary for transmission in chickens, further implicating quail in extending this virus to terrestrial poultry. Adaptation of the quail-adapted virus in chickens resulted in the alteration of viral tropism from intestinal shedding to shedding and transmission via the respiratory tract. Sequence analysis indicated that this chicken-adapted virus maintained all quail-adaptive mutations, as well as an additional change in the HA and, most notably, a 27-amino-acid deletion in the stalk region of neuraminidase (NA), a genotypic marker of influenza virus adaptation to chickens. This stalk deletion was shown to be responsible for the change in virus tropism from the intestine to the respiratory tract.
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Le document en format XML
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Sorrell</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</s1><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</wicri:noRegion><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Haichen Song" sort="Haichen Song" uniqKey="Haichen Song" last="Haichen Song">HAICHEN SONG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Veterinary Medicine, and Synbiotics Corporation,8075 Greenmead Drive</s1><s2>College Park, Maryland 20742</s2><s3>USA</s3><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>College Park, Maryland 20742</wicri:noRegion></affiliation></author><author><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</s1><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</wicri:noRegion><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</s1><s3>USA</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>University of Maryland, College Park, Virginia-Maryland College of Veterinary Medicine</wicri:noRegion><orgName type="university">Université du Maryland</orgName><placeName><settlement type="city">College Park (Maryland)</settlement><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno 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xml:lang="fr"><term>Animaux</term><term>Appareil respiratoire (virologie)</term><term>Caille</term><term>Coturnix</term><term>Données de séquences moléculaires</term><term>Délétion de séquence</term><term>Grippe chez les oiseaux (virologie)</term><term>Lignée cellulaire</term><term>Maladies de la volaille (virologie)</term><term>Poulets</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Réplication virale</term><term>Sialidase (génétique)</term><term>Sialidase (métabolisme)</term><term>Sous-type H2N2 du virus de la grippe A (enzymologie)</term><term>Sous-type H2N2 du virus de la grippe A (génétique)</term><term>Sous-type H2N2 du virus de la grippe A (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Sous-type H2N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Sialidase</term><term>Sous-type H2N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Neuraminidase</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales</term><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H2N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Grippe chez les oiseaux</term><term>Maladies de la volaille</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza in Birds</term><term>Poultry Diseases</term><term>Respiratory System</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chickens</term><term>Coturnix</term><term>Molecular Sequence Data</term><term>Quail</term><term>Sequence Deletion</term><term>Virus Replication</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Caille</term><term>Coturnix</term><term>Données de séquences moléculaires</term><term>Délétion de séquence</term><term>Influenzavirus aviaire</term><term>Lignée cellulaire</term><term>Poulets</term><term>Réplication virale</term><term>Virus grippal A</term><term>Poulet</term><term>Délétion</term><term>Mutation</term><term>Réplication</term><term>Voie respiratoire</term><term>Vétérinaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vétérinaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The events and mechanisms that lead to interspecies transmission of, and host adaptation to, influenza A virus are unknown; however, both surface and internal proteins have been implicated. Our previous report highlighted the role that Japanese quail play as an intermediate host, expanding the host range of a mallard H2N2 virus, A/mallard/Potsdam/178-4/83 (H2N2), through viral adaptation. This quail-adapted virus supported transmission in quail and increased its host range to replicate and be transmitted efficiently in chickens. Here we report that of the six amino acid changes in the quail-adapted virus, a single change in the hemagglutinin (HA) was crucial for transmission in quail, while the changes in the polymerase genes favored replication at lower temperatures than those for the wild-type mallard virus. Reverse genetic analysis indicated that all adaptive mutations were necessary for transmission in chickens, further implicating quail in extending this virus to terrestrial poultry. Adaptation of the quail-adapted virus in chickens resulted in the alteration of viral tropism from intestinal shedding to shedding and transmission via the respiratory tract. Sequence analysis indicated that this chicken-adapted virus maintained all quail-adaptive mutations, as well as an additional change in the HA and, most notably, a 27-amino-acid deletion in the stalk region of neuraminidase (NA), a genotypic marker of influenza virus adaptation to chickens. This stalk deletion was shown to be responsible for the change in virus tropism from the intestine to the respiratory tract.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region><settlement><li>College Park (Maryland)</li></settlement><orgName><li>Université du Maryland</li></orgName></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Sorrell, Erin M" sort="Sorrell, Erin M" uniqKey="Sorrell E" first="Erin M." last="Sorrell">Erin M. Sorrell</name></region><name sortKey="Haichen Song" sort="Haichen Song" uniqKey="Haichen Song" last="Haichen Song">HAICHEN SONG</name><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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